Combining Expression and Protective Efficacy of 45W-4BX and 18kD from Different Stages of Taenia solium

【Objective】The study aimed looked at the efficacy of the recombinant antigen of co-expressed 45W-4BX and 18kD from Taenia solium different development stages.【Method】 Truncated 45W-4B with the signal peptide sequence deletion and C’-terminal nucleotides encoding,17 hydrophobic amino acids were cloned into BamH I and EcoR I pre-digested pGEX-4T-1 and transformed into competent BL21.The positive transformants were determined by enzyme restriction,PCR,and sequencing.This was followed by ligating with the signal sequence.It removed the 18kD fragment and identified pGEX-4BX/18.In order to evaluate the protective efficacy of 4BX/18 and 4BX antigens,vaccination trail was performed with 300μg dose per pig.【Result】SDS-PAGE and Western blot showed that the 50kD fusion protein was expressed at a high level in E.coli and recognized by sera from either cysticercosis patients or in pigs infected by Cysticercus cellulosae.Statistical data indicated that the efficacy of the group immunized with 4BX/18 was 97% reduction in the number of cysticerci,a little higher than that of the GST-4BX group(95%).【Conclusion】 Recombinant antigens GST-4BX and GST-4BX/18 have high protection,supporting the development of highly efficient recombinant vaccines against cysticercosis,using protective antigens from different developmental stages of the parasite.