A MICROFLUIDIC PLATFORM FOR PERSONALIZED CANCER DIAGNOSTICS BY PADLOCK PROBES LIGATION AND CIRCLE-TO-CIRCLE AMPLIFICATION

We present a microfluidic system built from modular platform elements intended for detection of the seven most common mutations in the v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) gene by highly selective padlock probes ligation combined with circle-to-circle amplification (C2CA) and fluorescence detection. Padlock probes assays have been successfully established and the feasibility of the ligation-based procedure on a microfluidic format has been evaluated in PMMA chambers. The assays are currently being integrated in the microfluidic system described in this paper.