Diffusion from steady-state profile (DSSP) for low cost, low concentration measurement of diffusion

Here we present Diffusion from Steady-State Profile (DSSP), a simple, low-cost technique to measure the diffusivity of labeled proteins in hydrogels that are typically used for 3D cell culture. This is a steady-state technique which allows the use of long-exposure imaging, thereby enabling operation with low protein concentrations without the need for relatively expensive imaging equipment or immunoassays.