Histamine reduces ZO-1 tight-junction protein expression in cultured retinal microvascular endothelial cells

We examined ZO-1 protein content in cultured retinal vascular endothelial cells to test the hypothesis that histamine alters tight-junction-protein expression. Histamine (10 -9 –10 -4 M) causes a reversible concentration-dependent reduction of ZO-1 protein content, mediated by both H 1 and H 2 receptors. Histamine reduces ZO-1 expression within the time associated with increased paracellular permeability. Tight-junction-protein alterations may be a novel explanation for the mechanism by which vasoactive agents increase microvascular permeability.