Enhancement of anaerobic digestion effluent treatment by microalgae immobilization: Characterized by fluorescence excitation-emission matrix coupled with parallel factor analysis in the photobioreactor

The bacterial-microalgal consortium has been investigated to anaerobic digestion effluent (ADE) treatment in the photobioreactor (PBR). However, the high concentrations of nutrients reduced the ADE treatment efficiency and the transformation of organic pollutants in PBR was still unclear. In this study, two-sequencing batch PBRs were operated with suspended Microcystis aeruginosa (M. aeruginosa, SMA) and immobilized M. aeruginosa (IMA) to compare the ADE treatment performance. Fluorescence excitation-emission matrix coupled with parallel factor analysis (EEM-PARAFAC) was conducted to identify organics degradations. The results showed that the proportion of living M. aeruginosa cell (86.4%) in PBR (IMA) was highly significant (p   tyrosine-like substances > tryptophan-like substances. The removals of humic-like matters (94.05 ± 0.92%) and tyrosine-like matters (91.13 ± 2.49%) in PBR (IMA) were significantly (p < 0.01) higher than those in PBR (SMA). Notably, the average removals of nutrients in PBR (IMA) were significantly (p < 0.05) higher than those in PBR (SMA). This result verified that microalgae immobilization benefitted nutrients removals with 93.05 ± 1.45% of NH4+-N and complete PO43−-P removal in PBR (IMA). Moreover, the enrichment of functional genera Flavobacterium and Opitutus contributed to decreasing the organics loadings and strengthening the ADE treatment performance. Therefore, this study verified microalgae immobilization enhanced the actual ADE treatment. Additionally, fluorescent organic pollutants degradations were further evaluated by EEM-PARAFAC analysis in the bacterial-microalgal consortium.