Hen Ovarian Factors Influencing Neovascularization in Mice

Factors influencing neovascularization were separated from hen ovarian extracts. The lyophilized materials of extracts embedded in ethylenevinylacetate copolymer (Elvax 40) were implanted on the lateral wall of the sheath of M. rectus abdominis in mice. These implants induced inflammation and neovascularization in both a dose and time-dependent manner. Inflammation and neovascularization were characterized by invasion of blood cells and by capillary development into the tissue surrounding the implant. The acid mucopolysaccharide fraction of the extracts and .5 M NaCl fraction of the acid mucopolysaccharide fraction separated by a column of Dowex 1-X2 (Cl-form) showed strong neovascularizing activity without inducing severe inflammation. Results suggest that acid mucopolysaccharides are involved in neovascularization in hen ovaries.