Abstract 14505: An Early Transcriptomic Signature Predicts Poor Outcome After Out-of-hospital Cardiac Arrest

Introduction: Early prognostication is a major challenge in comatose survivors of OHCA. Hypothesis: We hypothesized that a wide analysis of the transcriptome could offer new prognostic tools and research axes. Methods: Patients were enrolled from an ancillary study of the clinical trial NCT00999583 that tested the effect of a high dose of erythropoietin (EPO) after OHCA. Blood samples were collected in comatose survivors of cardiac arrest at hospital admission and at days 1 and 3. Whole-blood RNA was purified, and processed to be hybridized on an Illumina HumanHT-12 V4 gene expression BeadChip, allowing a follow up of more than 34000 genes. Transcriptome profiles were compared between patients with favorable vs poor outcome (Cerebral performance category [CPC] 1-2 vs CPC>2) and treated or not by EPO (fold change >1.3; p<0.05). Results: Blood collection was performed in 69 patients. Among them, 33 and 36 patients were treated by EPO or not, respectively. The outcome was not different between these two grou...