Effects of activin A on extracellular matrix secretion of LX-2

Objective:To learn the effects of activin A on the secretion of extracellular matrix(ECM) in vitro cultured LX-2 cell.Method:Cultured LX-2 cells were divided into five groups.In addition to the normal control group,the rest four groups were added different concentrations of activin A 10,20,100 and 200 μg/L,respectively.The contents of amino-terminal peptide of procollagen type Ⅲ(PⅢNP),collagen type Ⅳ(Ⅳ-COL),hyaluronic acid(HA)and laminin(LN)in culture supernatants were detected by chemiluminescence after 24,48 and 72 h,respectively.Result:The secretion of PⅢNP,Ⅳ-COL and HA in HSC were enhanced by activin A according to its concentration(10-200 μg/L)in a time dose-dependent manner.Activin A had no effect on LN at 10-100 μg/L(P0.05),while significant difference was presented after 72 h in the 200μg/L activin A intervention group than that in the normal control group(P0.01).Conclusion:ACTA could induce HSC secrete ECM successfully.PⅢNP et al four indexes could reflect that ACTA might participate the occurrence and developing process of liver fibrosis and pathological degree.How to block ACTA way in the prevention and treatment of liver fibrosis provides us some new ideas.