In vivo synthesis of complex N-glycans by expression of human N-acetylglucosaminyltransferase I in the filamentous fungus Trichoderma reesei

Abstract The human N -acetylglucosaminyltransferase I gene was introduced in the genome of Trichoderma reesei strain VTT-D-80133. Expression was studied after induction from the cellobiohydrolase I promoter. Successful in vivo transfer of GlcNAc was demonstrated by analyzing the neutral N -glycans which were synthesized on cellobiohydrolase I. Final proof of the formation of GlcNAcMan 5 GlcNAc 2 was obtained by NMR analysis.