Дегидрогеназная активность сульфатредуцирующих бактерий как параметр оценки эффективности бактерицидов в нефтепромысловой отрасли

The comparative results of biocide effectiveness determination of 7 reagents with different active components in relation to sulfate-reducing bacteria (SRB) selected from waters of the Ust-Teguss oil field are presented. The biocide effectiveness of reagents was determined by two ways: by the method used at the present moment for the value of oil industry bactericide effectiveness (RD 39-3-973-83) and by change of the relative dehydrogenase activity of sulfate-reducing bacteria. The synthetic model of formation water (the source of selected SRB) with sodium lactate as an electron donor is proposed as a medium for the bacteria contact with the reagents. The similar results of biocide effectiveness determination of reagents by two different methods are obtained. The possibility of development a new method of biocide effectiveness determination of reagents based on measurement dehydrogenase activity of sulfate-reducing bacteria is shown. The evolution of dehydrogenase activity of sulfate-reducing bacteria is studied, it is shown their highest activity on the 2nd–3rd incubation days.