Identifying Human Papilloma Virus (HPV) in Women with Genital Warts by Multiplex–PCR Method

Article history: Received: 15 Nov 2012 Accepted: 12 Feb 2013 Available online: 11 May 2013 ZJRMS 2013; 15 (10): 45-48 Background: Many studies have shown that some types of 3THuman 3T Papillomavirus (HPV) play an important role in cervix cancer which has been identified in 99% of cervix cancers worldwide. This study aimed at identifying the frequency of high-risk types of this virus in cervical lesions among patients referred to Al-Zahra Hospital, Rasht. Materials and Methods: Forty five vaginal and cervical swap samples of women with genital warts were tested considering the presence of DNA of 14 common HPV types by using 3 series of specific PCR. Results: Out of 45 vaginal swap samples of women suffering genital warts, 37 samples (82.2%) were reported with 95% CI (70.6-93.83) considering the presence of HPV. The frequency distribution of HPV types in 45 patients with genital wart were as follows: 5 cases (13.5%) with HPV35, 4 cases (10.8 %) with HPV16, 4 cases (10.8 %) with HPV31, 3 cases (8.1 %) with HPV33, 3 cases (8.1 %) with HPV52, 3 cases (8.1 %) with HPV58, 2 cases (5.4 %) with HPV45, 1 case (2.7 %) with HPV18, 1 case (2.7 %) with HPV59, and 1 case (2.7 %) with HPV39. Conclusion: Inasmuch as samples of our study have been collected from one of the most important women-referring medical centers (Al-Zahra Medical and Teaching Center) in Guilan province, thus the results can be used for screening, management, and vaccination of the target population against the common types of virus in Guilan. Copyright © 2013 Zahedan University of Medical Sciences. All rights reserved.