Study of Bacterial Samples Using Laser Induced Breakdown Spectroscopy

Laser-induced breakdown spectroscopy(LIBS) technique has been applied to investigate two diflferent types of bacteria,Escherichia coli(B1) and Micrococcus luteus(B2) deposited on glass slides using Spectrolaser 7000.LIBS spectra were analyzed using spectrolaser software.LIBS spectrum of glass substrate was compared with bacteria spectra.Ca,Mg,Na,K,P,S,Cl,Fe,Al,Mn,Cu,C,H and CN-band appeared in bacterial samples in air.Two carbon lines at193.02 nm,247.88 nm and one hydrogen line at 656.28 nm with intensity ratios of 1.9,1.83 and1.53 appeared in bacterial samples Bl and B2 respectively.Carbon and hydrogen are the important components of the bio-samples Uke bacteria and other cancer cells.Investigation on LIBS spectra of the samples in He and Ar atmospheres is also presented.Ni lines appeared only in B2 sample in Ar atmosphere.From the present experimental results we are able to show that LIBS technique has a potential in the identification and discrimination of different types of bacteria.