The Biocompatibility on Medical High Nitrogen Nickel-Free Stainless Steel In Vitro

The biocompatibility of the austenitic stainless steel with different nitrogen contents was studied, The MTT(3-(4,5-Dimethylthi azol-2-yl)-2,5-diphenyltetra zolium bromide) assay, platelet adhesion and hemolysis rate test were used to examine the cytotoxicity. The cell compatibility of materials was investigated by cell adhesion test. Hemolysis rate test and platelet adhesion were used to examine the blood compatibility. The cell toxicity test showed that the nickel free austenitic stainless steel materials of different nitrogen content and the control group of titanium alloy materials had no significant side effects for vascular endothelial cells. The cell adhesion test showed that the cell number of vascular endothelial in the austenitic stainless steel was more than the titanium alloy materials, and the cells grow in good condition. The hemolysis rate was lower than 5%, and the influence of platelet was not significant, and pyrogen test results can be considered that such material extracts heat checking compliance with safety regulations.