Role of earthworm in degradation of soil phenanthrene by Pseudomonas putida

A 30-day incubation test was conducted to investigate the effects of treatments earthworm(E),bacteria(Pseudomonas putida)(B)and earthworm-bacteria(BE)on the degradation of soil phenanthrene.The degradation rate of soil phenanthrene at its initial concentration of 50 mg·kg-1 was in the sequence of BEBECK,and that at the concentration of 150 mg·kg-1 was 98.86% in BE,being significantly higher than that in CK and E.With the increase of the initial concentration of soil phenanthrene,the bacterial dioxygenase activity almost did not change in B,but increased significantly in BE.Under the same concentration of soil phenanthrene,the phenanthrene content in earthworm was significant higher in BE than in E,suggesting that earthworm could decrease the concentration of soil phenanthrene via its bioaccumulation,and the interaction between earthworm and P.putida could further promote the biodegradation of soil phenanthrene.