IN VIVO ANTIOXIDANT AND POTENTIAL ANTITUMOR ACTIVITY EXTRACT OF LEEA GUINEENSIS ROYEN EX. L. (LEEACEAE) ON CARCINOMATOUS CELLS

Summary The study was designed to investigate the subacute toxicity, in vivo antioxidant and antitumor activity of aqueous methanol extract of Leea guineensis (Leeacaea) (AMELG) on bearing carcinomatous cells. The results the toxicity study indicated no death of rats after 26 days of administration of the extract. At the concentrations of 500 mg/kg and 1000 mg/kg of the body weight, the liver enzymes activity alanine amino transferase (ALT) and aspartate amino transferase (AST) did not varied significantly as well as the level of glutathione. However, the concentration of creatinine and the activity of alkaline phosphatase (ALP) enzyme of the treated rats increased significantly from 57.26 to 62.33 (liver homogenate) and 89.87 to 92.52 (serum) compare to the control. The results of in vivo antioxidant activity revealed that the level of MDA decrease significantly (3.01 ± 0.05 – 2.17 ± 0.48) while the concentration of glutathione and the activities of CAT and SOD increased significantly after treatment with AMELG at 100 mg/kg and 200 mg/kg body weight. The results suggest that the aqueous methanol extract of Leea guineensis is not toxic and exhibits significant antioxidant and antitumor effects on bearing carcinomatous cells.