Voltage-driven counting of phospholipid vesicles with nanopipettes by resistive-pulse principle

Abstract We herein systematically investigate the voltage-driven counting of phospholipid vesicles with single conical nanopipettes by resistive-pulse principle in liquid phase. With increasing the positive bias voltage, the translocation-event frequency increases and the dwell time decreases. We analyze the effect of applied voltage on nanoparticle dynamics based on the calculation of transfer frequency and translocation velocity of the vesicle at the nanopipette orifice and find that the applied voltage shows an obvious influence on analytical performance and can serve as a useful parameter to optimizing the analytical performance of vesicles. This study demonstrates that the nanopipette could be used as an easily operated platform for vesicle counting.