Sporolactobacillus pectinivorans sp. nov., an anaerobic bacterium isolated from spoiled jelly
2016
A Gram-positive-staining, endospore-forming, facultatively anaerobic, lactic-acid-producing bacterium, strain GD201205T, was isolated from spoiled jelly in China. Strain GD201205T fermented glucose, fructose, mannose, sucrose, raffinose and turanose, but negative for nitrate reduction, catalase and oxidase. The predominant fatty acids of the strain were anteiso-C17 : 0 and anteiso-C15 : 0. Whole-cell hydrolysates contained glycine and alanine with meso-iaminopimelic acid as the diagnostic diamino acid. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, glycolipid 1 and glycolipid 2. The DNA G+C content of strain GD201205T was 48.7 mol%. 16S rRNA gene sequence analysis indicated that the strain belonged to the genus
Sporolactobacillus
and was most closely related to
Sporolactobacillus vineae
KCTC 5376T and
Sporolactobacillus putidus
JCM 15325T with 16S rRNA gene sequence similarities of 97.5 and 96.9 %, respectively. Levels of DNA–DNA relatedness between strain GD201205T and
Sporolactobacillus vineae
KCTC 5376Tand
Sporolactobacillus putidus
JCM 15325T were 29.2 and 47.6 %, respectively. Phylogenetic analysis based on the 16S rRNA gene and gyrB gene revealed that strain GD201205T was clearly distinct from all related species of the genus
Sporolactobacillus
. On the basis of the phylogenetic, chemotaxonomic and phenotypic evidence given in this study, strain GD201205T should be classified as a representative of a novel species of the genus
Sporolactobacillus
for which the name
Sporolactobacillus
pectinivorans is proposed. The type strain is GD201205T (=CICC 23867T=KCTC 15488T).
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