Isolation of Total RNA from Rice Endosperm and Expression of Important Genes Related to Grain Quality in Rice (Oryza sativa)

A simple method was advanced to wipe starch off RNA and obtain purer total RNA.This method was successfully applied in Northern blot and RNA array experiments to study the expression of starch branching enzyme gene (Rbe1) and Wx gene, which were important for starch synthesis. And the results indicate that on the whole, the expression of Wx gene was linear with amylose content of rice endosperm, and there were two Rbe1 homogenes in rice endosperm, the longer expressed only in endosperm and the shorter in all tissues.