SpyTag/SpyCatcher cyclization enhances the thermostability and organic solvent tolerance of l-phenylalanine aldolase

Yida Wang,Junwei Tian,Ying Xiao,Yue Wang,Hongxu Sun,Yanhong Chang,Hui Luo

SpyTag/SpyCatcher cyclization enhances the thermostability and organic solvent tolerance of l-phenylalanine aldolase

2019

Yida Wang
Junwei Tian
Ying Xiao
Yue Wang
Hongxu Sun
Yanhong Chang
Hui Luo

Objectives To improve the thermostability and organic solvent tolerance of L-phenylserine aldolase, the in vivo SpyTag/SpyCatcher cyclization strategy was applied in this work.

Keywords:

Thermostability
Biochemistry
Biology
Protein engineering
Aldolase A
Solvent
Phenylalanine
Stereochemistry
Bioconversion
Enzyme
Enzyme kinetics
Acetone

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