Liquid ready-to-use prothrombin time detection reagent

The invention discloses a liquid ready-to-use prothrombin time detection reagent. The liquid ready-to-use prothrombin time detection reagent comprises a buffer, a synthetic phospholipid, a recombinant rabbit tissue factor, a surfactant, and a stabilizing agent; the synthetic phospholipid is composed of phosphatidylserine, phosphatidylcholine, and cholesterol. According to a preparation method, the recombinant rabbit tissue factor and the synthetic phospholipid are adopted, the liquid ready-to-use prothrombin time detection reagent is prepared via selection on the composition of the synthetic phospholipid and optimization of the stabilizing agent; the liquid ready-to-use prothrombin time detection reagent is ready to use after decapping, and no redissolving is needed. The liquid ready-to-use prothrombin time detection reagent is high in stability and stability, and small in batch difference; the quality can be controlled easily; and production is convenient.