Multisubunit complex eEF1H in human glial tumors: from mRNA to protein

Aim. To investigate protein level of all subunits of the eukaryotic elongation translation factor eEF1H (eEF1A, eEF1Ba, eEF1Bb and eEF1Bg) in glial tumors of human brain in comparison with normal brain. Methods. The eEF1H components content has been investigated in human glioblastoma clinical samples by Western blot analysis. Results. To determine the eEF1Ba, eEF1Bb and eEF1Bg content, the polyclonal antibodies against all eEF1H subunits were obtained. The tendency of the eEF1Bg protein level to increase in glioblastomas was observed. There were no significant differences in the eEF1A, eEF1Ba and eEF1Bb protein contents. Conclusions. In the previous report we analysed the expression of all eEF1H subunits in human glial brain tumor on the mRNA level. This study showed that eEF1Bg was overexpressed while no significant changes in other eEF1H subunits were observed. It suggests a possible function of eEFBg which is cancer-related and is not connected with the functioning of eEF1H complex in translation.