PBP-1A affinity β- lactam antibiotics are within the electrochemical biosensor and its preparation method and application

The present invention discloses an affinity PBP-1A and the β- lactam antibiotics type electrochemical biosensor and its preparation method and application, characterized by a glassy carbon electrode surface with graphene, penicillin G and nafion common modifications, which method comprises preparing polishing and cleaning bare electrode and the activation step; graphene, penicillin G were added dropwise to a glassy carbon electrode surface, mixed, and then added dropwise at 37 ℃ Nafion dry, air dry surface, immersed in 5wt% bovine serum proteins, the oven 37 ℃, 30min, removed rinsed with PBS solution to obtain a surface-modified graphene - penicillin G-nafion glassy carbon as a working electrode, the working electrode was placed on the above-described system, the DPV detected, concentration of the standard curve determined in the test sample according β- lactam antibiotics, with the advantage of high sensitivity, speed, and low detection limits and efficient, inexpensive.