A method of extraction and separation of Agrocybe

The present invention belongs to the field of extracting and separating a polysaccharide is disclosed a method of extraction and separation of the Agrocybe, said step of extraction methods Agrocybe follows: (1) pre-cooled Chaxingu fresh cut into pieces, (2) after freeze dried tea tree mushroom slices quickly crushed, pulverized powder is added to an aqueous ethanol solution Agrocybe, ultrasonic stirring extraction, after extraction the extract was centrifuged, centrifugation; Agrocybe be frozen before freeze-drying supernatant; (3) the supernatant was concentrated, and concentrated to a weight of 2 times the weight of the Agrocybe; (4) the membrane filtration concentrate Chaxingu, the molecular weight of the membrane 1000, collecting the retentate; ( 5) was passed through a molecular weight cutoff of the membrane 200, collecting the retentate; (6) was concentrated to a solvent-free; (7) the concentrate was purified by crystallization from ethanol, filtered, and air-dried filter as a Agrocybe polysaccharide.