Heavy-Oil Anaerobic Degradation and Methanogenic Characterization

Heavy oil Anaerobic degradation by microbial had important theoretical significance and application value of reservoir residual oil biological gasification. The microbial consortium SLY-1 which was enriched from heavy oil reservoir in Shengli oilfield had been cultured at 55°C. DGGE showed that there were two isolated strains which were Anaeromyxobacter sp. and Anaerobaculum sp. respectively and three uncultured bacteria included in SLY-1. After 380d’s culture, SLY-1 had been produced 1006 μmol of methane accounting for 95.2% and 4.8% of other gases (butane, pentane, 2,2-two methyl butane hydrocarbon, 2-cyclopentane etc.). The rate of heavy oil degradation by microbial consortium SLY-1 reached 30.6%. The high viscous microbial consortium could lay the foundation for the residual low-grade heavy oil gasification.