Cloning and eukaryotic expression of 3C gene of OA58 strain of foot-and-mouth disease virus type O

The 3C gene of FMDV was amplified by RT-PCR,and then inserted into pMD18-T.Sequencing results showed that the amplified fragment was the whole encoding region of the 3C gene of FMDV.The plasmid pMD18-3C and the expression vector pEGFP-N1 were digested by BamHⅠ+XhoⅠ.The recombinant plasmid was identified by restriction enzyme analysis and PCR.Sequence analysis confirmed that the 3C gene was cloned into the expression vector successfully.The recombinant plasmid pEGFP-3C was transfected into BHK-21 cells.The expressed product was examined by fluorescent and RT-PCR.The results suggested that the 3C gene was expressed successfully in BHK-21.