Isolation of quaternary benzo[c]phenanthridine alkaloids from Macleaya microcarpa (MAXIM.) FEDDE: comparison of maceration, Soxhlet extraction and pressurised liquid extraction.
2012
Macleaya microcarpa (Papaveraceae family) has been of
considerable interest in recent years as a prospective source
of quaternary benzo[c]phenanthridine alkaloids (QBAs) related
to many pharmaceutical beneficial effects. For this purpose, a
quantitative, efficient and fast method to isolate the QBAs
from the plant material is required. To optimise and compare
pressurised liquid extraction (PLE) with Soxhlet extraction and
maceration in order to estimate extraction conditions for fast
and efficient isolation of QBAs contained in the roots of
Macleaya microcarpa. The QBAs were extracted by PLE, Soxhlet
extraction and maceration at different conditions (solvent,
time, etc.). Reversed phase HPLC with diode-array detector was
utilised for their determination and quantification. To
optimise the PLE procedure, the variable parameters, including
temperature (40C-150C), sample-to-inert material ratio,
extraction time (5-30 min) and number of extraction cycles
(1-4), were also tested. Quantitative determination of QBAs
resulted in 0.2-2.8 mg/g, 0.3-2.5 mg/g and 0.3-3.1 mg/g for
PLE, Soxhlet extraction and maceration. To produce the yields
mentioned above, PLE required only up to 30 min compared with
21 h for Soxhlet extraction and 49 days for maceration. PLE
provided an effective and fast extraction of QBAs fromM.
microcarpa roots and can be recommended as an alternative
isolation method to conventional techniques for QBAs from the
plant sources.
Keywords:
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
25
References
8
Citations
NaN
KQI