Virus influenza equina. Adaptación de aislados nacionales a cultivos celulares y aplicación de la prueba de seroneutralización

Chilean, strains of equine influenza virus halve different behavior in tissue cultures. Strain A/ equi/1/Santiago 77 (H7 N7) produces constant and specific cytopathic effect in MA-104, EFK and CEF cells. Strain A/equi/2/Santiago 85 (H3 N8) does not produce cytophatic effect in the same cells. Seroneutralization (SN) test was similar to hemagglutination inhibition (HAI) test when measure specific antibody titres against strain A/equi/I/Santiago 77 (H7 N7). Further more, SN test presents similar average titres, significant correlation and a similar sensitivity than HAI test. It is concluded that SN test may be used in diagnosis and research of equine influenza virus, strain A/equi/1/Santiago 77 (H7 N7), instead of HAI test with the additional advantage of measuring protective antibodies.