Construction and expression of ScFv against antibacterial peptide Magainin II

Tai-Shan Hu,Haixu Chen,Yun-Gui Yang,Youcun Qian,Denghong Chen,Xian-ming Qu,Sheng-li Yang

Construction and expression of ScFv against antibacterial peptide Magainin II

2000

Tai-Shan Hu
Haixu Chen
Yun-Gui Yang
Youcun Qian
Denghong Chen
Xian-ming Qu
Sheng-li Yang

SDS-PAGE and western-blot analyses showed the ScFvs were secretingly expressed in E. coli HB 2151. The result of indirect ELISA showed the soluble and phage displayed ScFv had antigen binding activity.

Keywords:

antigen binding
Molecular biology
Chemistry
indirect elisa
antibacterial peptide
Magainin

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