Selection of potentials for pulsed amperometric detection of carbohydrates at gold electrodes

Pulsed amperometric detection of carbohydrates requires that the carbohydrates be oxidized at the detection potential and that the responsivity of the working electrode be restored during the cleaning, and conditions pulses. Selection of the potentials for detection, cleaning, and conditioning should be based on the voltammetric behavior of the Au working electrode and the carbohydrates to be detected in the mobile phase used for the separation. A method for the selection of the pulse potentials and its application to the detection of a mixture of monosaccharides are presented