Investigation on the relationship of apolipoprotein A5 gene-3A/C polymorphism with blood lipid metabolism and relevant diseases

Objective To investigate the relationship of apolipoprotein A5(apoA5) gene-3A/G single nucleotide polymorphism(SNP) with blood lipid metabolism and relevant diseases,and provide the data for the clinical disease diagnosis and early intervention.Methods The blood samples were collected from 232 healthy Han people,209 coronary heart disease(CHD) patients,132 diabetes mellitus(DM) patients,107 cerebral infarction(CI) patients and 199 hypertension(HTN) patients in Shanghai Jing′an District Central Hospital.DNA extraction,polymerase chain reaction(PCR) amplification and DNA sequencing were used to detect apoA5 gene-3A/G SNP.The biochemical analyzers were used to detect the levels of blood lipid such as triglyceride(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-C) and low density lipoprotein cholesterol(LDL-C).Their relationship was analyzed statistically.Results AA homozygote,AG heterozygote and GG homozygote were 52.5%,39.7% and 7.8% respectively.The distribution sequences of A and G alleles were 72.4% and 27.6%.Compared the healthy Han people group of GG gene type to ones of AA and AG gene types,TG raised(P0.05),HDL-C reduced(P0.05) and non-HDL-C(non-HDL-C) raised(P0.05).Compared the control group to the disease group,the value of TG had statistical significance in the gene types of AA and AG(P0.05),and the value of TG in the control group of GG gene type raised lightly with no statistical significance(P0.05).Conclusions The regulation from apoA5 gene-3A/G SNP to the disease of blood lipid metabolism is mainly performed at the relationship between gene type of GG and high level of TG,low level of HDL-C and high level of non-HDL-C.This gene is one of the candidate genes which leads to the disorder of lipid metabolism.