Transcriptome analysis of zebra fish (Danio rerio) eggs following treatment with malachite green

Abstract Malachite green is widely used in aquaculture as a parasiticide and in food, textile, and other industries for other purposes. Most previous studies have reported harmful effects as phenotypes; however, few studies have explored the mechanism of malachite green's toxicity. In the present study, the effect of malachite green on the eggs of the model fish zebra fish (Danio rerio) eggs were analyzed at the transcriptome level to study the toxicity mechanism of malachite green on aquatic organisms. Six normalized cDNA libraries were constructed using RNA from the control group and the malachite green treatment group. A total of 257,730,582 clean reads were obtained. In comparison with the reference genome, statistical analysis of the reads, unmapped reads, multimap reads, and the multimap read rate showed no significant differences (p > .05). However, the mapped read rate and unmapped read rate showed significant differences (p