A Preliminary Analysis of Gene Expression of Seven Isoforms of ADP-glucose Pyrophosphorylase(AGPase)in Rice Endosperm under Different Temperature Conditions

Different temperature treatments(33℃ and 25℃ of daily mean temperature for high and normal temperature treatments,respectively) and the real-time fluorescence quantitative PCR(FQ-PCR) were used to analyze the expression patterns of seven isoforms(AGPS1,AGPS2a,AGPS2b,AGPL1,AGPL2,AGPL3 and AGPL4) of ADP-glucose pyrophosphorylase(AGPase) which was the key enzyme in starch synthesis metabolism in rice endosperm with two rice varieties Teqing and Thai Fragrant Rice as the materials.The results showed that the three isoforms of AGPS2b、AGPL2、AGPL3 had much higher expression amounts than the other four isoforms,thus they were thought to be the main expression patterns of AGPase in rice endersperm.The relative expression amounts of AGPL2 was the highest among all isoforms.The relative expression amounts of AGPS2b、AGPL2、AGPL3 were higher in the normal temperature treatment than in the high temperature treatment in both rice varieties.The relative expression amounts of the three enzyme genes at all tested milking stages in Teqing were higher than those in Thai Fragrant Rice under different temperature treatments.