Protocol for Somatic Embryogenesis in Passiflora cincinnata Mast. (Passifloraceae)

The process of somatic embryogenesis has become an essential asset, as it enables plant regeneration and large-scale propagation. Our research team has pioneered a reproducible protocol for somatic embryogenesis using mature zygotic embryo of P. cincinnata. Here, we describe in details the protocol for P. cincinnata, in which the explants were exposed to medium supplemented with 2,4-dichlorophenoxyacetic acid and 6-benzyladenine. Due to the efficacy and reproducibility of this regeneration protocol, new perspectives arise as the protocol can be extended to other Passifloraceae species that arouse agronomic, ornamental and commercial interest. Moreover, this is an alternative for genetic transformation protocols, based up to now on the organogenic system.