Dynamics of enzymes generating hydrogen peroxide in solid-state fermentation ofPanus tigrinus on wheat straw

The relationship between the production of extracellular H2O2, hydrogen peroxide-producing enzymes and ligninolytic peroxidase was examined during solid-state cultivation ofPanus tigrinus on wheat straw. Glyoxal oxidase, Mn2+-dependent peroxidase and glucose oxidase, capable of H2O2 generation, were found in the extracellular enzyme preparation. The production of H2O2 has two maxima: the maximal production correlates well with the maximal activities of glyoxal oxidase and Mn2+-dependent peroxidase, while another, lower peak of H2O2 generation is related to the second peak of Mn2+-dependent peroxidase activity. The contribution of glucose oxidase to the production of hydrogen peroxide is probably only marginal. Comparison of the dynamics of these extracellular activities and the ligninolytic peroxidase showed good temporal correlation indicating an interrelation of the two processes.