Improved paclitaxel production by fed-batch suspension cultures of Taxus chinensis in bioreactors

Feeding sucrose at 20 g l -1 on day 16 gave maximum paclitaxel production at 10 mg l -1 when Taxus chinensis in 5 I bioreactors, Paclitaxel accumulation was doubled by the cultivation of cells initially with dissolved O 2 tension at 60% for 20 days followed by being at 20% for another 12 days in the bioreactor. Combination of these two strategies gave maximum paclitaxel production of 19 mg l -1 after 32 days.