Polymorphism of GnRHR gene and its relationship with litter size trait of Saanen Dairy Goat.

Detected polymorphism of GnRHR Gene in Saanen Dairy Goat,and ascertained the relationship between polymorphism of GnRHR Gene and the litter size trait.The gonadotropin releasing hormone receptor gene(GnRHR) was studied as a candidate gene for the prolificacy of Saanen Dairy Goat.Seven pairs of primers were designed to detect single nucleotide polymorphisms of exon 1 and exon 2 of the GnRHR gene.Only the products amplified by primers P3 and P7 displayed polymorphisms.For primer P3,two genotypes(AA and AB) were detected in Saanen Dairy Goat and Boer Goats,revealed two mutations(+714-point loss of the base A and +731 base G insertion) of exon 1 in genotype AB compared with genotype AA.For primer P7,two genotypes(CC and CD) were detected in Boer Goats,only one genotype CC was detected from Saanen Dairy Goat,revealed one mutations(+1305C→G) of exon 2 in genotype CD compared with genotype CC.The Saanen Dairy Goat with homozygous genotype AA have 0.31(P0.05) kids less than those heterozygotes type AB.