The Effects of Non-Small Cell Lung Carcinoma Conditioned Media on Skeletal Muscle Cell Proliferation and Metabolism

Lung cancer is the second most common cancer and one of the largest causes of cancer-related mortality worldwide. 80–85% of all new lung cancer diagnoses are Non-Small Cell Lung Carcinoma (NSCLC), suggesting a great need to continue elucidating the metabolic mechanisms surrounding this prevalent disease. Due to the oncogenic environment surrounding NSCLC growth, factors released into the environment could have a direct impact on other tissues. In particular, skeletal muscle, a leading insulin-sensitive and metabolically active tissue, is subject to wasting in many cancer patients. The purpose of this study was to determine how NSCLC conditioned media affects C2C12 skeletal muscle cells proliferation and metabolism. C2C12 mouse skeletal muscle cells were grown for 24 hours and serum-starved overnight with serum-free media to induce quiescence. Cells were treated with equal volumes of either fresh growth media or conditioned media from proliferating NSCLC cells. C2C12 myoblast proliferation was assessed eve...