The Screening of Optimal Ganoderma lucidum and the Effect of Medium Substrate on Bioactive Metabolite in Submerged Culture

To determine an optimal G. lucidum strain, we made the production of mycelia biomass, EPS, IPS, IT, ET as indicators, among the five tested strains, the American G. lucidum was screened, and the production of mycelia biomass, EPS, IPS, IT, ET could reach 2.013g/100 ml, 11.2988 mg/100ml, 23.7800 mg/10ml, 45.5412 mg/100ml, 11.1417 mg/100ml, respectively. On the other hand, according to the screening of carbon and nitrogen sources as well as their concentration, the suitable carbon and nitrogen was 3% malt powder, 1.5% yeast extract. So we can use the fermentation culture for the following research, which was as follows：3% malt powder, 1.5%yeast extract, 0.3% KH2PO4, 0.15% MgSO4, 0.005% VB1.