A Smartsystem based on a Lab on a Card for detection of Salmonella spp. In faeces

This work describes a SU-8 microdevice for the detection of Salmonella spp. This device is capable of: (i) concentrating the target microorganisms from human faecal samples, (ii) lysing them and (iii) amplifying the extracted DNA by real time Polymerase Chain Reaction (q-PCR). All the steps are carried out in a single 1.5 mul microchamber in 25 minutes. Unlike previous reported PCR devices, this work describes a membrane valve compatible with the PCR chamber.