Effects of capsaicin on induction of c-jun proto-oncogene expression in Fisher-344 rats by N-methyl-N′-nitro-N-nitrosoguanidine

Abstract N -Methyl- N ′-nitro- N -nitrosoguanidine (MNNG) is a potent inducer of cellular stress leading to chromosomal aberrations, point mutations, and cell death. To study the effect of capsaicin on c- jun expression when given with MNNG to rats, Fisher-344 rats that had been administered MNNG were treated with capsaicin in their diet and organs were removed for measuring c- jun transcripts. We show that pre- or post-treatment of capsaicin relative to MNNG administration up- or down-regulates (depending on the organ) c- jun expression in a consistent pattern in most organs. In fact, we found in this study that capsaicin inhibits c- jun induction, stimulated by MNNG, in the spleen, heart, stomach and lung. Since MNNG, a methylating agent, is a powerful carcinogen that is very effective in the induction of c- jun mRNA, the results suggest that capsaicin uptake in the diet could play a role in inhibition of tumorigenesis induced by MNNG.