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Primers to detect Plasmodium

2008 
A method for detecting or identifying an infection with Plasmodium genus and / or one or more of entrePlasmodium falciparum, Plasmodium vivax, Plasmodium malariae and Plasmodium ovale in a sample; the method comprising the following steps (a) to (c): a) extracting DNA from the sample; b) amplifying a particular region of a gene sequence of 18S rRNA of Plasmodium using procedure laid LAMP reacting the DNA extracted in step (a) in a reaction mixture quecontiene a DNA polymerase strand displacement and a set of specific primers desecuencia ; c) detecting or identifying the presence or absence of an amplified the genus Plasmodium, amplificadoen step (b) product in which the set of sequence-specific primers is a deoligonucleotidos array containing the nucleic acid sequences represented by SEQ ID : 1 to 6 I paraamplificar a particular region of a gene sequence of 18S rRNA of Plasmodium, and optionally detecting or identifying the presence or absence of an amplified one or more of entrePlasmodium falciparum, Plasmodium vivax, Plasmodium malariae and Plasmodium ovale product amplified in step (b), wherein the set of sequence-specific primers is a set of oligonucleotides contienesecuencias nucleic acid represented by SEQ ID NO: 1 to 6 for amplifying a particular region unasecuencia gene 18S rRNA Plasmodium and one or more of a primer set comprising unconjunto oligonucleotide containing sequences aci nucleic do represented by SEQ ID NO: 7 to 12 paraamplificar a particular region of a gene sequence of 18S rRNA of Plasmodium falciparum, one decebadores assembly comprising a set of oligonucleotides containing acid sequences nucleicorepresentadas by SEQ ID NO: 13 18, SEQ ID NO: 31-36 or SEQ ID NO: 37 to 42 to amplify a region particularde a gene sequence of 18S rRNA of Plasmodium vivax, a primer set comprising unconjunto oligonucleotide containing nucleic acid sequences represented by SEQ ID NO: 19 to 24 paraamplificar a particular region of a gene sequence of 18S rRNA of Plasmodium malariae and decebadores assembly comprising a set of oligonucleotides containing acid sequences nucleicorepresentadas by SEQ ID NO: 25 to 30 to amplify a particular region of a sequence of rRNA gene 18Sde Plasmodium ovale.
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