G0 Cell Cycle Arrest Alone is Insufficient for Enabling the Repair of Ionizing Radiation-Induced Potentially Lethal Damage

Abstract van Bree, C., Rodermond, H. M., ten Cate, R., de Vos, J., Stalpers, L. J. A., Haveman, J., Medema, J. P. and Franken, N. A. P. G0 Cell Cycle Arrest Alone is Insufficient for Enabling the Repair of Ionizing Radiation-Induced Potentially Lethal Damage. Radiat. Res. 170, 184–191 (2008). The repair of ionizing radiation-induced potentially lethal damage (PLD) is suggested to be important for the clinical response to radiotherapy. PLD repair is usually studied in quiescent cultures prepared by growing cells to confluence with an accumulation of cells in G0 phase of the cell cycle, but the biological pathways enabling PLD repair are still unknown. In this study, we examined whether the controlled expression of two different inducers of G0 cell cycle arrest, the human tumor suppressor gene growth arrest specific 1 (GAS1) in murine fibroblasts and the forkhead transcription factor FOXO3a in human colon carcinoma cells, is sufficient to enable PLD repair. We found that GAS1 and FOXO3a induced a cell cycle...