Mitigation of Acetamiprid – Induced Renotoxicity by Natural Antioxidants via the Regulation of ICAM, NF-kB and TLR 4 pathways

Abstract Background Acetamiprid (ACMP) is a member of the neonicotinoid group of insecticides. It is extensively used worldwide. The misuse of ACMP creates danger hazards to human and animal. Methods ACMP induced renal damage evidenced by an increase in kidney injury biomarkers. So the goal of this work is to clarify the reno protective effect of Quercetin(Qrctn) and/or Nano-glutathione (N- Gluta) solely or in combination to counterbalance the danger effect of ACMP. All treatments with the previous agents were coadministered orally with ACMP for one month. Results ACMP ingestion caused a significant rise in serum creatinin, urea, and uric acid, TNF α along with renal cystatin C, lipid peroxidation and nitric oxide with the concomitant decline in the levels of reduced glutathione and IL-10 levels. Protein expression of ICAM was upregulated as well as mRNA expression of NF-kB while mRNA expression of Nrf2 was down-regulated. Immune histochemistry of TLR 4 revealed strong Immune reaction. The administration of Qrctn or N- Gluta either individually or together modulated all the preceding aforementioned parameters. Conclusion; Fascinatingly Qrctn and N- Gluta combination was the most powerful regimen to frustrate ACMP reno-toxicity and may be deliberate as a hopeful applicant for renal therapy.