Effects of HECCM and DDPH on the expression of osteopontin gene and α-SM-actin in PASMC

AIM:To study the effects of hypoxial endothelia cell conditional medium(HECCM) and DDPH on the expression of osteopontin(OPN) gene and α-SM-actin in pulmonary arterial muscle cell(PASMC),and the reverse effect of DDPH on proliferating PASMC induced by HECCM.METHODS:Hypoxial endothelia cell model was established in PASMC of swine.MTT assay and Immunocytochemistry was used to test cell proliferation,the expression of α-SM-actin.Reverse transcription polymerase chain reaction(RT-PCR) was used to detect the expression of OPN gene.RESULTS:(1)HECCM decrease the level of α-SM-actin and up-regulate the expression of OPN gene in PASMC;(2)DDPH increase the level of α-SM-actin and down-regulate the expression of OPN gene in PASMC.CONCLUSION:DDPH could reverse the phenotype transformation of PASMC exposed to HECCM,and transform PASMC from synthetic phenotype to normal contractile phenotype.