Coexpression of α1,2 galactosyltransferase and UDP-galactose transporter efficiently galactosylates N- and O-glycans in Saccharomyces cerevisiae

Mami Kainuma1, Nobuhiro Ishida2, Takehiko Yoko-o1, Shigemi Yoshioka2, Makoto Takeuchi3, Masao Kawakita2 and Yoshifumi Jigami1,4 1Department of Molecular Biology, National Institute of Bioscience and Human Technology, Tsukuba, Ibaraki 305–8566 Japan, 2Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoken), Tokyo 113–8613 Japan and 3Central Laboratories for Key Technology, KIRIN Brewery Co., Yokohama, Kanagawa 236–0004, Japan