Differential immunomodulatory effect of vitamin D (1,25 (OH)2 D3) on the innate immune response in different types of cells infected in vitro with IBDV

Abstract It has been demostrated that vitamin D (Vit D) included in diets offers a beneficial effect by improving innate immune responses in chickens. However, its mechanisms of action and the effect on immunosupresive pathogens, such as infectious bursal disease virus (IBDV), are not yet known. In the present study, we have studied the immunomodulatory effect of Vit D on the innate immune response in three cell lines; Fibroblast cells (DF-1), Macrophages (HD11) and B cells (DT-40) infected with IBDV (Intermediate vaccine) at two multiplicity of infections (MOI) (1 and 0.1). Genes associated with innate immune responses (TLR-3, TLR-21, MDA-5, MyD88, TRIF, IRF-7, INF-α, INF-β, PKR, OAS, viperin, IL-1β, IL-6 and IL-12) were evaluated at different time points (3, 6, 12, 24 and 36 hours post infection, h.p.i). Virus production reached a maximum at 24 h.p.i., which was significantly (p