Characterization of microsatellite loci markers for the critically endangered Montseny brook newt (Calotriton arnoldi)

Fifteen novel tetranucleotide polymorphic microsatellite loci are described and characterized for the critically endangered Montseny brook newt Calotriton arnoldi. Until now little is known about genetic variability and dispersal of this species across its very limited distribution area, mainly represented by two main sectors (eastern and western from the Tordera river valley). The level of genetic diversity of the new loci was assessed in 23 individuals from the eastern sector. Number of alleles per locus ranged from 2 to 8 (mean = 4.7) and observed and expected heterozygosity ranged from 0.087 to 1.000 and 0.162 to 0.814, respectively. Almost all loci were found to be in Hardy–Weinberg equilibrium and were not linked. These polymorphic loci can be used in population structure, gene flow, and population differentiation. The value of such information should be high for successful management and conservation of this potentially highly threatened species.