Antioxidant and cardioprotective effects of Ilex brasiliensis: A comparative study with Ilex paraguariensis (yerba mate)

Abstract Our objective was to compare the antioxidant properties and cardiovascular effects in ischemia–reperfusion of leaves aqueous extracts of Ilex brasiliensis (B) and Ilex paraguariensis (P). In vitro systems were used to assess the antioxidant properties of the extracts. Isolated rat hearts were treated with both extracts before ischemia and myocardial function was assessed. Thiobarbituric acid reactive substances (TBARS) concentration and reduced glutathione (GSH) were also measured. B extract showed higher total phenols and ascorbic acid contents and a higher scavenging activity of peroxynitrite and ferric reducing antioxidant power (FRAP) than P extract. Postischemic systolic and diastolic functions were improved after B and P treatment. Both extracts decreased TBARS and preserved GSH content. The present study demonstrates that an aqueous extract of I. brasiliensis , similar to I. paraguariensis , protects the myocardium against ischemia–reperfusion injury and attenuates oxidative damage. These effects may be attributed to the potent antioxidant properties of the extract.