The Role of CLAMP Binding Sites in Maintaining of Distant Interactions in Drosophila Transgenic Lines

The mechanism of specific recruitment of the dosage compensation complex (DCC) to the X chromosome of male Drosophila remains an unresolved problem. Recently, a DNA-binding protein CLAMP, which binds to the primary chromosome entry sites (CESs) of the DCC, has been described as a factor involved in X chromosome recruiting of DCC. It has been shown that CESs can recruit DCC to autosomes. In this work, we studied the ability of a well-described CES to support distant interactions in model transgenic Drosophila lines. Negative results were obtained showing that the CES that is bound by CLAMP protein does not support distance interactions.