System establishment of plant virus-free regeneration from explants of Amorphophallus konjac

Explants were leaf-stalk segments by detection.Small leaf-stalk segments were inoculated in M1-M12 12 kinds of media.M8(MS+6-BA 0.5 mg/L+NAA 0.1 mg/L)medium was suitable for callus induction,the rate of induction was 100 %;Callus of leaf-stalk segments were inoculated in M8-M12 5 kindsof media.M9(MS+6-BA 1.0 mg/L+NAA 0.1 mg/L)medium was suitable for differentiation of buds,the rate of differentiation was 66.7 %;Efficiency seedlings were inoculated in M8-M14 7 kinds of media.M11(MS+6-BA 1.5 mg/L+NAA 0.15 mg/L)medium was suitable for formation of roots,the root growth rate reached 100 %.